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发布于:2020-9-2 00:11:35  访问:63 次 回复:0 篇
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Sential for pEIB202 transfer with a significantly (P < 0.001) decreased (by 2? log
Putative lipoprotein p007 was also proved to be important for pEIB202 transfer PF-04965842 Protocol pubmed ID:https://www.ncbi.nlm.nih.gov/pubmed/28570310 (Fig. It was intriguingly to find that p007 and topA are involved in the conjugation transfer. We further asked whether deletion of these genes in pEIB202 would affect the expression of virB genes, which subsequently modulates the T4SS function and activities to influence plasmid conjugation processes. We (-)-(S)-Equol Estrogen Receptor/ERR carried out qRT-PCR to test the expression of virB9 and virB4, encoding two core proteins for T4SS function, in WT, p007 and topA strains as well as their complement strains. TheOutput/input a 0.015 0.0058 0.00099 0.0022 0.0039 0.0014 0.0034 0.10 0.011 0.18 0.0027 0.026 0.0086 0.019 0.073 4.30 0.020 0.13 0.028 0.Table 4 Genes identified by PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/24895203 TIS in pEIB202 that affects maintenance or conjugation of pEIBLoci ETAE_p001 ETAE_p002 ETAE_p003 ETAE_p004 ETAE_p005 ETAE_p006 ETAE_p007 ETAE_p008 ETAE_p009 ETAE_p010 ETAE_p011 ETAE_p012 ETAE_p013 ETAE_p014 ETAE_p015 ETAE_p016 ETAE_p026 ETAE_p047 ETAE_p048 ETAE_paGene virD4 virB11 virBProduct T4SS component VirD4 T4SS component VirB11 T4SS component VirB10 hypothetical proteinP-value 0.000548801 0.000531746 0.000531259 0.000517762 0.000520783 0.000530141 0.000493853 0.0003746 0.000544597 1.61153E-05 0.000535705 0.0003982 0.Abrocitinib custom synthesis 000541165 0.000514194 9.96994E-06 0.000340276 6.77947E-06 0.000639706 0.000545018 2.38855E-virB9 virBT4SS component VirB9 T4SS component VirB8 putative lipoprotein hypothetical proteinvirBT4SS component VirB6 putative lipoproteinvirBT4SS component VirB5 hypothetical proteinvirB4 virBT4SS component VirB4 T4SS component VirB2 hypothetical proteintopADNA topoisomerase transcriptional repressor proteinmobC virD2 traCputative mobilisation protein VirD2 component DNA primase TraCThe data indicate that the read number of a specific gene in output is significantly under-represented or over-represented as compared to that in input with a cutoff of less than 0.25 or higher than 4-fold and p < 0.Liu et al.Sential for pEIB202 transfer with a significantly (P < 0.001) decreased (by 2? log) output. The gene topA, encoding a DNA topoisomerase I, was the only candidate that inhibiting pEIB202 transfer (Table 4) (Fig. 3a). Insertions in the TA loci in this gene caused 4.30-fold increase (P < 0.001) in the output reads. All the regions related to the above-described 20 genes shared averaged input reads, suggesting that these genes were not associated with the maintenance or replication, but conjugation transfer in E. piscicida.In order to further verify the TIS identified genes as the regulators or modulators controlling pEIB202 transfer, we constructed in-frame deletion mutants and corresponding complement strains for topA, p007, and virB9 and determined the transfer efficiency of the mutated pEIB202. The mutant with a deletion in virB9, encoding the channel component protein essential for T4SS function [32], showed significantly reduced transfer frequency (Fig. 3b), suggesting that, as expected, the proper function of T4SS is required for pEIB202 horizontal transfer. Putative lipoprotein p007 was also proved to be important for pEIB202 transfer PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/28570310 (Fig. 3b). The plasmid without gene topA has a significantly higher transfer frequency in comparison to that of the WT (Fig.
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